Polygalacturonase Activity and Ethylene Synthesis during Cucumber Fruit Development and Maturation

In vitro synthesis and processing of tomato fruit polygalacturonase.

The in vitro processing of tomato fruit polygalacturonase (PG) (poly[1,4-alpha-d-galacturonide]glucanohydrolase, EC 3.2.1.15) was studied. Complete chemical deglycosylation of a mixture of mature, purified PG 2A and PG 2B isozymes (45 and 46 kilodaltons; respectively) with trifluoromethane sulfonic acid yielded a single polypeptide of 42 kilodaltons. Similarly, N-terminal amino acid sequencing ...

Alcohol Acetyltransferase Activity, Ethylene Production and Aroma Formation of Muskmelon during Fruit Development

Muskmelon (Cucumis melo L.) varieties differ in a range of physical and chemical attributes. Aroma is one of the most important factors determining fruit quality and consumer preference. Volatile esters, a major class of compounds contributing to the aroma of muskmelon fruit, are synthesized by alcohol acyl-transferases (AATs). We demonstrate here that volatile aldehydes are most abundant in un...

Inhibitor-resistant early ethylene production during tomato fruit development

In addition to the ethylene formed at the onset of tomato fruit ripening, three peaks of ethylene are produced during earlier periods of in vitro development of tomato flower to fruit. This is the first report characterizing ethylene production during early development of tomato fruit. Previous reports from this laboratory showed that VFNT Cherry tomato calyces are transformed into fruit tissue...

Profiling of Volatile Compounds and Associated Gene Expression and Enzyme Activity during Fruit Development in Two Cucumber Cultivars

Changes in volatile content, as well as associated gene expression and enzyme activity in developing cucumber fruits were investigated in two Cucumis sativus L. lines (No. 26 and No. 14) that differ significantly in fruit flavor. Total volatile, six-carbon (C6) aldehyde, linolenic and linoleic acid content were higher during the early stages, whereas the nine-carbon (C9) aldehyde content was hi...

Polygalacturonase in normal and abnormal tomato fruit.